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This textbook presents more than 250 current problems from modern biophysics and related fields of application, together with detailed solutions. The problems emphasize concepts and information that are essential for an understanding of the fundamental physical processes in life sciences, including medical science. The book is divided into 11 chapters and the topics follow the sequence of dimensions and diversity of the living world. It starts with the basic principles relating to the energetics of the living world, and moves on to problems from the microworld (biophysics of molecules and membranes) and the macroworld (biomechanics, biophysics of organs, radiation and the environment). The subsequent chapters are devoted to problems concerning the application of different experimental methods in biophysics and in medicine (diagnostic and therapeutic methods and medical imaging). The problems in biostatistics help the reader to understand and to digest the concepts and the methods of evaluation of experimental data. The book is written for undergraduate and graduate students, with a view to their improving problem-solving ability in physics. The reader is faced with the great challenge of finding solutions to the problems, but at the same time his or her knowledge of important concepts and relations is reinforced. As the level of difficulty of the problems covers a wide range, both beginners and advanced readers will find pleasure in the book.